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KMID : 1094720070120040399
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 4 p.399 ~ p.403
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Adaptation of Chinese hamster ovary cells to high potassium ion-containing medium for enhancement of follicle-stimulating hormone production
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Yoon Sung-Kwan
Jeong Myeong-Hyeon
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Abstract
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In this study, a suspension culture of recombinant Chinese hamster ovary (CHO) cells producing follicle-stimulating hormone (FSH) was used to investigate the effects of potassium ion (K+) on cell growth and FSH production. Cell growth was significantly suppressed at a K+ concentration higher than 60 mM, but specific FSH productivity (q FSH) was enhanced more than 2-fold compared to the value obtained at 4 mM K+. In an attempt to alleviate the cell growth suppression at a high K+ concentration, the cells were adapted at 60 mM K+ in a repeated batch mode. During adaptation, the growth rate increased from 0.010 to 0.020 h?1, andq FSH also gradually increased and reached 11.1 ng/(106 cells h), which was even higher than that of the unadapted cells at 60 mM K+. The adapted cells showed a 2.6-fold increase in maximum FSH titer at 80 mM K+ compared to the unadapted cells at 4 mM K+. Taken together, these results demonstrate the potential of using culture media containing cells adapted to high K+ concentrations, for the enhancement of recombinant protein production.
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KEYWORD
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potassium ion, adaptation , CHO cells, follicle-stimulating hormone
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